University of Washington Department of Microbiology
Standard Operating Procedures for Chemicals or
Processes
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#1
Process (if applicable) |
Inorganic Bases - handling, dispensing, and diluting including: potassium hydroxide,
ethanolamine,triethanolamine, sodium hydroxide |
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#2
Chemicals |
Bases cause burns to
skin and eyes upon contact and to mucous membranes if inhaled or ingested. |
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#3
Personal Protective
Equipment (PPE) |
Wear chemical splash
goggles. For concentrated bases use heavy duty neoprene gloves or natural
rubber gloves. A lab coat or apron is recommended for personal protection and
is required when dispensing quantities greater than 1 liter or when cleaning
up a spill of a quantity greater than 1 liter. |
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#4
Environmental / Ventilation Controls |
Concentrated bases
should be dispensed in a fume hood. |
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#5
Special Handling Procedures & Storage Requirements |
When diluting bases,
small amounts should be added gradually to water and mixed thoroughly to dissipate
any heat generated. Bases should be stored separately from acids, oxidizers,
and flammable solvents. Bases in glass bottles over 1 liter should be
transported in spill proof carriers. Bases are stored 352 base cabinet of
hood. |
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#6
Spill and Accident
Procedures |
In case of skin
contact, flush affected areas with copious amounts of water for 15 minutes.
Obtain medical attention. Neutralize any spilled base with citric acid. Spill
kit can be found next to bookcase in 352. |
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#7 Waste
Disposal |
Small quantities may
be neutralized by adding water. Bases greater than or equal to pH 12.5 are a
hazardous waste and should be labeled with a 'Hazardous Waste Label'. Contact
EH&S at 5-2848 for collection. |
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#8
Special Precautions
for
Animal Use (if applicable) |
* NA |
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Particularly hazardous substance involved? |
YES: |
Blocks #9 to #11 are Mandatory |
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X NO: |
Blocks #9 to #11 are Optional. |
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#9
Approval Required |
N/A |
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#10 Decontamination |
N/A |
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#11 Designated Area |
N/A |
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Name: Title: |
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Signature: Date:
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Environmental Health and Safety, Box 354400 *to
be filled in by PI or Supervisor