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University of Washington School of Medicine, Department of Microbiology     

Ten Golden Rules of Tissue Culture

1.   No gloves in regular trash.

2.   Seal off vacuum when done.

3.   Empty vacuum trap flask when full.

4.   Fill bleach bottle when empty

5.   Clean hemocytometer after use.

6.   No liquid in biohazard bag.

7.   Put glass pipettes fully in sharps container.

8.   Fold new pipette boxes when full.

9.   Discard full pipette boxes.

10.   Keep opened tip boxes sterile

 

 

 

 

 

 

For specifics on appropriate handling and waste procedures please see the online chemical SOPs or our waste and spill notebook located in room 352.

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